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Enrichment of Supercritical Extracts in beta-Sitosterol by Fractionation Process
Rochová, Kristina ; Sovová, Helena ; Sajfrtová, Marie ; Bártlová, Milena
The aim of this contribution was to use the extraction with supercritical CO2 coupled with fractionation of extract for preparation of extracts rich in beta-sitosterol. Phytosterols have been proved to lower serum cholesterol levels in humans and to have other benefit for human health. Supercritical extraction is a green method to separate such compounds from plants. Sea buckthorn seeds and sunflower seeds were extracted and a mathematical model was applied to determine separation factor of beta-sitosterol as minor component of extract. According to the obtained results, conditions of fractionation were set up. The most important enrichment of a part of extract from seeds with beta-sitosterol was achieved with fractionation in two separators (first separator kept at 15MPa and 50°C) and subsequent fractionation in time at the output of the second separator.
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Extrakty z rostlin jako směsi a jejich předběžné dělení superkritickým CO2
Sovová, Helena ; Sajfrtová, Marie ; Rochová, Kristina
This work concerns supercritical extraction from plants, a semi-continuous process where solutes are extracted from a fixed bed of plant particles to a stream of supercritical CO2. Though the efficiency of control of extract composition in this one-equilibrium-stage process is lower than in the counter-current column, the composition of extract is affected substantially by extraction conditions. Further equilibrium stages are represented by the following separation of extract from solute in two or more separators adjusted to different pressures and temperatures, where the least soluble components precipitate first.
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Supercritical Extraction and Fractionation of Sea Buckthorn Oil: Beta-Sitosterol Selectivity to Oil
Rochová, Kristina ; Sovová, Helena
Sea buckthorn (Hippophae rhamnoides L.) contains large amounts of compounds important in the human diet and used in medicine, among others phytosterols, whose anticancer, anti-inflammatory and antibacterial effects have been proved. Phytosterols, particularly beta-sitosterol, occur in sea buckthorn oil. The oil was extracted from ground seeds with supercritical carbon dioxide at the temperatures 40 and 60 oC and pressures 15-28 MPa. Using HPLC analysis for beta-sitosterol, its selectivity to the oil was evaluated. The results enable us to increase the concentration of beta-sitosterol in the extract.
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